FITC anticorps (Alkaline Phosphatase (AP))

N° du produit ABIN401116

Cet anticorps Chèvre Polyclonal détecte spécifiquement FITC dans WB, IHC (fro), EIA et ID. Il présente une réactivité envers .

Aperçu rapide pour FITC anticorps (Alkaline Phosphatase (AP)) (ABIN401116)

Antigène: FITC (Fluorescein Isothiocyanate (FITC))

Reactivité: Veuillez nous consulter SVP

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp FITC est conjugé à/à la Alkaline Phosphatase (AP)

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunodiffusion (ID)

Détail du produit anti-FITC anticorps (Alkaline Phosphatase (AP))

Purification: Affinity Chromatography

Immunogène: Fluorescein conjugated to Goat IgG

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1/2,000 - 1/10,000. Western Blot: 1/500 - 1/2,500. Immunohistochemistry: 1/200 - 1/: 1,000. Immunodiffusion.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.05 M Tris Chloride, 0.15 M Sodium Chloride, 0.005 M Magnesium Chloride, 0.0005 M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0 containing 10 mg/mL BSA (IgG and Protease Free) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: DO NOT FREEZE!!

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur FITC

Antigène: FITC (Fluorescein Isothiocyanate (FITC))

Autre désignation: FITC

Classe de substances: Chemical

Sujet: Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is? pH sensitive and is significantly reduced below? pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.Synonyms: Fluorescein Isothiocyanate